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bir3 domain  (R&D Systems)


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    Structured Review

    R&D Systems bir3 domain
    Bir3 Domain, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bir3+domain/Recombinant+Human+XIAP+(BIR3)+Protein%2C+CF/us11542492-1196-26-39
    Average 91 stars, based on 4 article reviews
    bir3 domain - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Binding Assay:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Recombinant:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    FP Assay:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).



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    Image Search Results


    The interaction network between the N -terminal tetrapeptide (Ala-Val-Pro-Ile) of Smac and the XIAP BIR3 domain (based on the NMR data) ( a ) and representation of the peptide-binding groove of the BIR3 domain containing the bound N-terminal AVPI motif of Smac (based on 1G73.pdb) ( b )

    Journal: Investigational New Drugs

    Article Title: Structure-based design, synthesis, and evaluation of the biological activity of novel phosphoroorganic small molecule IAP antagonists

    doi: 10.1007/s10637-020-00923-4

    Figure Lengend Snippet: The interaction network between the N -terminal tetrapeptide (Ala-Val-Pro-Ile) of Smac and the XIAP BIR3 domain (based on the NMR data) ( a ) and representation of the peptide-binding groove of the BIR3 domain containing the bound N-terminal AVPI motif of Smac (based on 1G73.pdb) ( b )

    Article Snippet: Phosphate buffer (pH 7.5) supplemented with γ-globulin (100 µg/mL) and NaN 3 (0.02% w/v ), fluorescent probe 4 and the XIAP BIR3 domain (Sino Biological, Beijing, China) were used for all measurements.

    Techniques: Binding Assay

    Determination of the binding affinity of fluorescent probe 4 and the XIAP BIR3 domain. The assay was performed at a constant fluorescent probe concentration (2 nM) and serially diluted XIAP BIR3 domain (ranging from 2.5 µM to 0.0763 nM)

    Journal: Investigational New Drugs

    Article Title: Structure-based design, synthesis, and evaluation of the biological activity of novel phosphoroorganic small molecule IAP antagonists

    doi: 10.1007/s10637-020-00923-4

    Figure Lengend Snippet: Determination of the binding affinity of fluorescent probe 4 and the XIAP BIR3 domain. The assay was performed at a constant fluorescent probe concentration (2 nM) and serially diluted XIAP BIR3 domain (ranging from 2.5 µM to 0.0763 nM)

    Article Snippet: Phosphate buffer (pH 7.5) supplemented with γ-globulin (100 µg/mL) and NaN 3 (0.02% w/v ), fluorescent probe 4 and the XIAP BIR3 domain (Sino Biological, Beijing, China) were used for all measurements.

    Techniques: Binding Assay, Concentration Assay